investigate the possibility of sex determination of rainbow trout (oncorhynchus mykiss) using molecular markers
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abstract
the study investigates the possibility of sex determination of rainbow trout (oncorhynchus mykiss)using molecular screen for products monosex of populations. 35 caudal fin tissue samples wereobtained from karaj rainbow trout mature population and then genomic dna was extracted andmarker loci amplified. polymerase chain reaction (pcr) was setup based on two omyfa andomyfatu markers for amplification of sex linked markers in rainbow trout. finally, the populationwas screened based on presence of a specific band for both markers. result of this expriments showedthat presence of omyfa and omyfatu markers in males were %100 and %55 respectively. acordingto the result omyfatu is proper marker for screen of karaj trout population.
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Journal title:
شیلاتجلد ۶۸، شماره ۱، صفحات ۱-۱۱
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